《植物生理学报》 2014, 50(8): 1209-1215

高温胁迫下不同光强对‘赤霞珠’葡萄PSII活性及恢复的影响

孙永江, 杜远鹏, 翟衡*

山东农业大学园艺科学与工程学院, 山东泰安271018

通信作者：翟衡；E-mail: zhaih@sdau.edu.cn；Tel: 0538-8241335

摘　要：

本文研究了高温与不同光强结合处理对‘赤霞珠’葡萄叶片PSII活性及恢复的影响。结果表明, 高温黑暗处理(40℃, 0 μmol•m^-2•s^-1)导致叶片PSII最大光化学效率(F_v/F_m)、反应中心吸收的光能用于电子传递的量子产额(Ψ_Eo)与单位反应中心光能的传递(ET_o/RC)降低明显, 且无恢复趋势, K点相对荧光(V_k)、单位反应中心光能的吸收(ABS/RC)与捕获(TR_o/RC)显著升高。高温弱光处理(40 ℃, 200 μmol•m^-2•s^-1)后的叶片PSII活性明显恢复, ET_o/RC降低明显, TR_o/RC无显著变化。高温强光(40 ℃, 1 600 μmol•m^-2•s^-1)处理导致单位面积有活性反应中心数量(RC/CS_m)抑制程度最大, 恢复程度较低。实验结果说明, 高温处理下黑暗对葡萄PSII功能活性及恢复均会造成抑制, 而弱光可以显著缓解高温对葡萄叶片的胁迫作用, 并促进PSII的恢复, 强光导致胁迫下的PSII功能抑制最明显。

关键词：葡萄; 高温; 光; 光抑制; 恢复; 光系统II

收稿：2014-03-26   修定：2014-06-28

资助：现代农业产业技术体系专项资金(CRAS-30)和长江学者和创新团队发展计划(IRT1155)。

Effects of Different Light Intensity on PSII Activity and Recovery of Vitis vinifera cv. Cabernet Sauvignon Leaves under High Temperature Stress

SUN Yong-Jiang, DU Yuan-Peng, ZHAI Heng*

College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, China

Corresponding author: ZHAI Heng; E-mail: zhaih@sdau.edu.cn; Tel: 0538-8241335

Abstract:

The effects of different light intensity on PSII activity of ‘Cabernet Sauvignon’ leaves under high temperature stress were investigated by analyzing chlorophyll a fluorescence transient and chlorophyll quenching simultaneously. The results showed that, compared with pre-treatment, treatment of high temperature and dark (40 ℃, 0 μmol•m^-2•s^-1) decreased maximum quantum yield for primary photochemistry (F_v/F_m), quantum yield for electron transport (Ψ_Eo) and electron transport flux per reaction center (ET_o/RC) significantly. The treatment also increased increased relative fluorescence intensity of K point (V_k), absorption flux per reaction center (ABS/RC), trapped energy flux per reaction center (TR_o/RC). There was no significant recovery after the heat and dark treatment. The treatment with high temperature and low light (40 ℃, 200 μmol•m^-2•s^-1) decreased ET_o/RC and did not change TR_o/RC significantly. After the treatment, PSII activity recovered to a great degree. And the treatment with high temperature and high light (40 ℃, 1 600 μmol•m^-2•s^-1) decreased density of reaction centers (RC/CS_m), while the recovery of PSII activity was not significant. The experiments showed that under high temperature treatment, dark treatment could inhibit both PSII activity and recovery, low light could alleviate the process significantly, while high light could inhibit PSII activity the most obviously under stress.

Key words: grape (Vitis vinifera); high temperature; light; recovery; PSII